Sentence examples for using a diagnostic pcr from inspiring English sources

gambiae complex by using a diagnostic PCR (6 ).

Our aim in this study was to determine if there were genetic sequences that are unique to the AES-I that might be used to develop a diagnostic PCR assay to enable rapid detection of this strain directly from CF patient sputum.

Therefore, all obscura group flies were collected and a diagnostic PCR assay was used to distinguish the species.

Independent clones were screened for homologous integration by PCR, using diagnostic PCR products for the presence of target gene genomic DNA and knockout vector sequences, as well as a diagnostic PCR product only found for homologous intergrant for both N and C-terminal regions of the knockout cassette.

Using the diagnostic PCR a total of 266 litters was positive for the PRRS virus.

First, each individual was karyotyped using a molecular PCR diagnostic that spans the inversion breakpoints (White, Santolamazza, et al. 2007).

We verified that all experimental aphid lines used in this study were free of facultative symbionts by using 1) diagnostic PCR to screen for all known pea aphid facultative symbionts, 2) 'mostly universal' PCR primers that amplify most bacteria, but not the obligate symbiont Buchnera, and 3) Denaturing Gradient Gel Electrophoresis (DGGE) with universal 16S rRNA bacterial primers.

Individual cultures from all of the available UW stocks were tested using the diagnostic PCR and none were found to have incurred the deletion (table 2).

In summary, although ABCB1 is validated as β-catenin target gene in a cell line model with defined β-catenin genotypes, the facile detection of β-catenin mutations using the diagnostic PCR procedure reported here is not sufficient for predicting therapy response or for individualisation of chemotherapy regimens for patients.

funestus group were molecularly distinguished using diagnostic PCR assays [ 18].

Proper integration of the constructs at the pan-2 locus was confirmed using diagnostic PCR with pan-2 flanking region and gene-specific primers.