23 Concentrations of HDL-C were measured with Roche Diagnostics-USA kits (Roche, Indianapolis, IN) after precipitation of apoB-containing lipoproteins using a dextran sulfate and magnesium chloride solution (Acros Organics, Morris Plains, NJ).
Visualisation was performed using a dextran polymer-conjugated horseradish-peroxidase and 3,3′-diaminobenzidine chromogen followed by counterstaining with hematoxylin solution (Gill 3, Sigma Aldrich, Munich, Germany).
Dextran concentrations in the lower chambers were determined using a dextran standard curve.
In brief, a brain homogenate was prepared, using a buffer and a dextran solution.
Blood was mixed with a dextran solution (3% (w/v) Dextran 500 in 0.9% NaCl, both Sigma Aldrich) by gentle inversion and left at room temperature for 20 min for sedimentation of red blood cells.
Thus, we expect to obtain efficient separation of colloidal particles in foods using highly concentrated dextran solutions.
The authors suggest increasing stromal thickness to 400 microns by avoiding dehydrating the cornea using an alternative riboflavin solution without dextran after epithelium debridement.
Briefly, islets were isolated using a discontinuous Dextran-based gradient.
