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The amount of gemcitabine was measured by UV spectrophotometer (Shimadzu, Japan) using a detection wavelength of 270 nm.
The amount of gemcitabine was measured by UV spectrophotometer using a detection wavelength of 270 nm (Blanco and Alonso 1997).
The chromatographic column used was a C18 column (5 μm 150 × 4.6 mm ID - VYDACTM, WR Grace & Co , USA with a flow rate of 0.7 mL/min, a loop of 20 μL and using a detection wavelength equal to λ= 210 nm [46].
HPLC was performed at 40 °C using a detection wavelength of 205 nm, a flow rate of 1.0 ml/min and an injection volume of 10 μl.
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For quantification, a microplate spectrophotometer was used with a detection wavelength of 490 nm for the MTS assay and detection at 490 nm with reference to 405 nm for the BrdU assay.
The HPLC assay was conducted using a UV detection wavelength of 259 nm.
The DAD was used for HMF and furfural with a detection wavelength of 280 nm and a reference wavelength of 360 nm.
Absorbance values of the lysates were determined on a Multiskan Ascent microplate reader (Thermo Labsystems, Helsinki, Finland), using a background wavelength of 620 nm and a detection wavelength of 550 nm.
Plates were read using a fluorescence plate reader (FLUOstar OPTIMA; BMG LABTECH) with an excitation wavelength of 485 nm and a detection wavelength of 530 nm.
To improve the current glucose detection limit of the sensor simple measurements using a reference wavelength (i.e., with no glucose absorption) or an out-of-phase excitation at a reference wavelength could be envisaged.
A Beckman 125 HPLC instrument (Beckman, USA) equipped with a Hamilton auto sampler and a 168 photodiode array detector (DAD) was used and the detection wavelength was set at 254 nm.
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