Later on, Tan et al. [ 72] reported a PPA detection method using a variational level set approach.
In Table 2 is presented a summary of agreement between experiments for the detection of expression (using a significance level of < 0.01) for the analysis-group (see also Additional File 1, Section 7).
The use of tag-eGFP fusion proteins allowed us to use eGFP detection levels as internal control.
In some experiments, ICB samples were treated with morphine (1, 10 and 100 μM) for 1 h and later examined for changes in NO levels using a NO detection kit.
The GSB detection is done using a pixel-level statistical approach proposed by [37].
Double samples of arterial blood were collected in gas-tight tubes and kept at 4°C until CO analysis, which was performed using an ultra-trace level gas detection system (RGA3 Reduction Gas Analyzer, SAES Getters, Milan, Italy), as described elsewhere (Tarquini et al., 2009).
Thorough experiments performed for the fabricated device show that the absolute detection error is less than 5%, and by quantifying the detected voltage using software, the minimum detection level is 8 ppm and the detection sensitivity is 9 ppm within the detection range of 8 1000 ppm.
We then preformed a t-test on the log2 ratio of each cDNA probe to identify genes discriminating PRE and POST samples from each patient by using a P-value < 0.05 detection level.
In contrast, we never found any Wolbachia within the male gonad by using a direct propidium iodide detection at any level observed, neither in the germ cells, before or during meiosis starting at this stage (Fig. 6D F) nor in the proximal somatic gonad, the vas deferens (Fig. 6G; supplementary material Movies 8 10).
FEC were performed using a modified McMaster technique with minimum detection level of 20 nematode eggs per gram (EPG) faeces [ 14].
