Sentence examples for using a density gradient from inspiring English sources

This resource includes detailed protocols describing blood collection tubes, isolation of PBMC using a density gradient, cryopreservation of PBMC, and thawing of cells as well as preparation for functional assays.

Peripheral Blood Mononuclear Cells (MNCs) were isolated using a density gradient as previously described [19].

Each gradient was fractionated into 40 fractions using a Density Gradient Fractionation System (Brandel, Gaithershurg, MD).

PBMC were almost immediately isolated from fresh blood in 49 children using a density gradient.

Semen samples are processed within one hour after ejaculation, using a density gradient centrifugation followed by washing with culture medium and is subsequently used for insemination.

PBL were harvested using a density gradient centrifugation kit (NycoPrep 1.077A, Axis-Shield, Oslo, Norway), washed several times and resuspended in PBS.

For the latter studies, the authors used a density gradient centrifugation technique to isolate progenitor cells from either the hypothalamus or the hippocampus, and they were able to detect many GnRH-immunoreactive cells in the cultures originated from both CNS regions.

Samples were prepared using a density-gradient separation of BM or PB cells (Ficoll-Paque; GE Healthcare, Milan, Italy).

To confirm this result, we used a density-gradient centrifugation-based cell fraction assay to separate the whole-cell extracts into the NO, NP, and cytoplasm.

Moreover, because we are pre-enriching for PM through the use of a density gradient we need be less concerned about differential partitioning of PM from endomembane based upon their relative solubility in the PEG and dextran phases.

The pancreas is then excised whole and placed in modified HBSS for stationary digestion at 37°C for 8 11 min as described in detail in Appendices A and B. There is some debate regarding the use of a density gradient to purify islets from acinar tissue.