Sentence examples similar to using a defocus µm from inspiring English sources

Images were taken under low-dose conditions at a magnification of 67,000X using a defocus value of −1.5 µm and recorded on DITABIS digital imaging plates (Pforzheim, Germany).

Data acquisition was done on an FEI Polara G2 microscope operated at 300 kV under low-dose conditions (28 e−/Å) using a defocus range of 1.6 4.0 μm.

A dataset of ∼1000 micrographs was acquired for the T20S in a single session using a defocus range between 0.9 and 2.4 µm.

Low-dose conditions during imaging (dose ∼25 e−/Å) were used for all data sets, and micrographs were collected using a defocus range of −0.6 to −1.5 μm.

Images were recorded on a 4k × 4k Gatan Ultrascan CCD camera using a defocus ranging from 0.5 to 1.5 μm and a dose of 25 e−/Å.

Analyses of carbon aggregates using a defocused electron microprobe beam reveal compositions similar to estimated partial melts.

For electron tomography of resin-embedded sections, dual-axis tilt series were collected with SerialEM (Mastronarde, 2005) at 2° increments over a range of ± 66° and using a 2 µm defocus.

The final solution was sterilized using a 0.22  µm filter.

Tissue samples were sliced using a cryostat at 30 µm (brains) or 12 µm (eyes).

For the patient with retinitis pigmentosa, we used a 50 µm × 50 µm ROI for quantification of photoreceptors at the border of photoreceptor atrophy.

In general, we used a 40×40 µm counting frame, a 1 µm guard, 100×100 µm sampling grid, and a dissector height of 8 µm.