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Images were quantified by ImageJ (http://rsb.info.nih.gov/ij/) using a defined region of interest (ROI), 2(b), and 2(c)).
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In this way, the input pattern to the IFTA algorithm calculating the holographic phase profile can be quickly generated using gray-level threshold detection, once a defined region of interest in the image is selected.
A 0.5 s pulse of the 405 nm laser at 50% power within a defined region was used to photo-convert mEos2.
Here, using a defined set of parameters, a region-of-interest was imaged with high-transmission, multi-wavelength excitation.
Platelets were identified by particle size using a previously defined region that includes >95% of CD41-positive events and the mean fluorescent intensity of 10 000 events was recorded.
For this reason, and based on previous studies implicating the rIFG in similar face-processing tasks [13], [43] [45], we conducted a subsequent between-group analysis focusing specifically on the right prefrontal cluster, using a functionally defined region-of-interest (ROI) derived from the group mean of the TD children in the Task-Rest contrast.
Therefore, the field is moving toward finer sampling of the fMRI scans [ 40, 62] and using functionally defined regions [ 63] to obtain a better picture of the local and global functional changes.
Uniquely, EMAGE searches can be made using spatially defined regions, as well as standard text based querying.
Similarly, an attempt to purify key factors from nuclear extracts using defined regions of Xist identified only hnRNPC, a generic mRNA processing factor (Brown and Baldry, 1996).
The venous images were assessed using defined regions of interest (ROIs) derived from the delayed planar image.
In analysis using defined regions, we found no significant region-specific changes in volume (Fig. 4G) or surface area (data not shown).
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