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To determine whether these two forms can interchange, we performed competition assays under steady-state conditions using a decreased amount of protein (0.1 μM).
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Ultimately it depends on the funder whether they are content with the transaction, yet it is undeniably part of a music culture using a decreasing amount of fiat money, and could further casualise music-making.
Tests using a decreasing amount of tissue were performed by dividing slides into areas containing 75%, 500 %and25%5 % of square polygons that were used to compute the Morisita index (Additional file 1).
Same observations were made for the images reconstructed using MAR, however with a decreased amount of metal- and beam hardening artifacts (Fig. 4c and d).
*The child is not drinking fluids, or is producing a decreased amount of urine.
Kaplan-Meier curves of the Morisita index estimated using decreasing amount of tissue.
(B ) TRAP assay of whole cell extracts from GFP, F-TERT, TR, or F-TERT+TR overexpressing hESCs using decreasing amount of protein (250, 50, 10, 2 ng).
(F ) TRAP assay of whole cell extracts from WT and promoter mutation-containing hESCs (day 147) using decreasing amount of protein (200, 40, 8 ng).
(A ) and (B ) TRAP assay of whole-cell extracts from cancer cell lines (HeLaS3, 293T, and HCT116), WT hESCs, and the NPCs or fibroblasts carrying the TERT promoter mutations (24 days after differentiation for fibroblasts and 20 days for NPCs) using decreasing amount of protein (200, 40, 8 ng).
In another test using decreasing amounts of tissue, the Morisita index remained prognostic in Her2+ cancer with as little as 50%% of tissue (Additional file 1, Additional file 2: Figure S9-10).
Although, because of its role in lead poisoning, it is no longer used as a pigment in paints, white lead is still used, though in decreasing amounts, in ceramics.
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