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Cytotoxic effects were analyzed by measurement of lactate dehydrogenase release using a cytotoxicity detection kit (Roche, Basel, Switzerland).
Lactate dehydrogenase (LDH) release was assayed using a Cytotoxicity Detection Kit PLUS (Roche, Mannheim, Germany), which evaluates cytotoxicity by measuring the amount of LDH released from the cytosol of damaged cells according to the manufacturer's protocols.
LDH activity was measured using a Cytotoxicity Detection kit (Roche Molecular Biochemicals, Indianapolis, IN, USA) and quantified by measuring absorbance at 490 nm.
The toxicity in cell cultures was examined routinely for lactate dehydrogenase leakage using a cytotoxicity detection kit (Roche Applied Science, Mannheim, Germany).
Neutrophil lysis either after incubation with CCY culture supernatant or following phagocytosis of serum opsonized USA300 was measured by the release of lactate dehydrogenase (LDH) using a Cytotoxicity Detection Kit (Roche Applied Sciences, Pleasanton, California) as described previously [23], [26].
Cytotoxicity levels were determined by measuring the amount of Lactate dehydrogenase (LDH) released into the culture media using a Cytotoxicity Detection Kit (Roche 11644793001, Roche Diagnostics GmbH Roche Applied Science Mannheim, GE) by ELISA according to the manufacturer's protocol.
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LDH activity was determined by the use of a cytotoxicity detection kit purchased from Roche Applied Science (Indianapolis, IN, USA).
The degree of platelet adhesion was determined using the lactate dehydrogenase (LDH) released when the adherent platelets were lysed with a Triton-PBS buffer (2% v/v Triton-X-100 in PBS) using a Roche Cytotoxicity Detection Kit (LDH).
Lysis of PMNs following exposure to filter-sterilized BHI culture supernatants was determined by release of lactate dehydrogenase (LDH) using a kit (Cytotoxicity Detection Kit, Roche Applied Sciences) as recommended by the manufacturer and as described [23], [24].
The cytotoxicity effect of CG was measured using an LDH cytotoxicity detection kit (Roche Applied Science, USA).
Cytotoxicity of pamidronate was determined by the release of lactate dehydrogenase from the cytosol of damaged cells using an LDH cytotoxicity detection kit (Roche Diagnostics, Mannheim, Germany).
More suggestions(15)
using a multichannel detection
using a spike detection
using a digoxigenin detection
using a peak detection
using a wavelet detection
using a polymer detection
using a Light detection
using a cytotoxicity cell
using a cytotoxicity luminescence
using a cytotoxicity assay
using a chemiluminescence detection
using a cytotoxicity endpoint
using a luciferase detection
using a chemiluminescent detection
using a contour detection
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