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In addition, antiviral activity against Chikungunya (CHIKV) virus was evaluated using a cytopathic effect inhibition assay.
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A homogenized biopsy sample was used to infect Vero cells, where a cytopathic effect typical of orthopoxviruses was seen after 2 days.
We used recombinant IFNα2 that was functional both in vitro, as determined by a cytopathic effect inhibition assay, and in vivo as evidenced by increased expression of CD69 on lymphocytes at 18 hr upon i.p. administration.
Infected cells were incubated until a cytopathic effect (CPE) was observed in 80%% of the monolayer, and collected supernatants were used for the next passage.
Delta-39, Delta-24 induced a cytopathic effect with similar efficiency in glioma cells, a comparable capacity for replication.
A cytopathic effect (CPE) reduction assay was adopted for screening the antiviral activities of the pure compounds isolated from the roots of E. jolkinii in the present study.
Cells were incubated at 37°C until a cytopathic effect was evident, then lysates were frozen and thawed twice.
Cultures were observed for 3 4 weeks for a cytopathic effect.
WT genome induced a cytopathic effect (cpe), indicative of productive infection, in both cell types.
The virus induced a cytopathic effect morphologically and temporally typical of a herpesvirus (data not shown).
Only sample 51 displayed a cytopathic effect after 72 h and was further analyzed.
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