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The serum levels of interleukins (IL-1β, IL-2, IL-6, IL-10, IL-12p70, and IL-17A), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in the experimental mice were determined using a cytometric bead array (CBA) kit.
Interleukins IL-6 and IL-8 were measured using a cytometric bead array (R&D Systems, Abingdon, UK) as previously described [28].
Cytokines were quantified using a cytometric bead array (CBA) mouse inflammation kit from BD Bioscience.
Supernatants were collected at baseline, 1, 2, 4, 6, 8, 12, 24 and 48 hours and assayed for inflammatory cytokines using a cytometric bead array.
Murine IFN-γ, IL-6, IL-10, IL-12p70, IL-17A, MCP-1 and TNF-α were measured using a cytometric bead array (CBA) kit according to the manufacturer's instructions (BD Biosciences).
Media from PBMC cultures stimulated for 24 h with 2 µg/ml phytohemagglutinin (Invitrogen) were calibrated against an IFNγ standard (National Cancer Institute, Frederick, MD) using a cytometric bead assay (BD Biosciences, San Jose, CA).
Similar(29)
Cytokines and chemokines in urine were evaluated using a Cytometric-Bead-Array-Flex-Set.
Levels of IFNγ, IL-2, IL-4, IL-6, IL-10 and TNFα were analysed in the serum or in cell culture supernatants using a mouse cytometric bead array Th1/Th2/ Th17 cytokine kit (BD Biosciences) following the manufacturer's instructions.
TNF, IL-2, IL-6, IL-5, IL-4, Granzyme B, MCP3 and IP-10 levels measured using a TH1/TH2 cytometric bead array (CBA) kit (Becton Dickinson) or a custom Luminex panel (Panomics).
After 24 h, cytokines were measured in the plasma using a multiplexing cytometric bead array and ELISA.
Cytokine secretion was assessed by flow cytometry using a BD Cytometric Bead Array (CBA) Mouse Inflammation Kit (Becton Dickinson).
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