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Full scan data was acquired by scanning from m/z 100 1000 in MS2 mode, using a cycle time of 0.5 s, with a step size of 0.1 µs.
A total of 79 transitions were monitored during a single LC-SRM run, using a cycle time of 0.5 s and a minimum dwell time of 10 ms. All data analyses were carried out using Pinpoint software (v.1.3).
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Exercise consisted of an 8-week conditioning program using a cycle ergometer (three times a week, 1 h session).
A parallel column format was used, giving a cycle time of 34 min. Tandem MS was performed by monitoring precursor to product transitions under individually optimized conditions, typically from the most abundant [M+nH]n+ precursor ion to an ammonium ion.
The models extrapolate the results of the ATTRACT trial for infliximab from 54 weeks to a life-time horizon using a cycle length of six months.
The tracer response in a closed, continuously circulating system is a limiting case of the tracer response in an open system with recycle, and this response can be used to define a cycle time distribution (CTD).
Mass spectra were also collected using flow-injection analysis of fractions of the HPLC eluate, using an injection cycle time of 0.8 min.
We found it necessary to use a higher cycle time because of display errors that occurred with consecutive writes at the fastest timing.
The balance between advantages and disadvantages by using short cycle time thus should be considered.
The relative mRNA expression level of each gene in each sample was calculated using comparative cycle time as described previously [57].
All specimens were then thermocycled between 5°C and 55°C for 500 cycles using a dwell time of 10 s and a transfer time of 30 s between each bath.
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