Sentence examples for using a cycle sequencing from inspiring English sources

To analyse end sequences, BAC DNAs were amplified using a TempliPhi large construction kit (GE Healthcare, UK), and the end sequences were analysed according to the Sanger method using a cycle sequencing kit (Big Dye-terminator kit, Applied Biosystems, USA) with DNA sequencer type 3730xl (Applied Biosystems).

Sequencing was performed using a cycle sequencing kit (Big Dye-terminator Cycle Sequencing kit, Applied Biosystems) with DNA sequencer type 3730xl (Applied Biosystems) or DeNOVA-5000HT (Shimadzu Co., Japan) according to the protocols recommended by the manufacturers.

PCR products were sequenced by using a cycle sequencing reaction with fluorescent dye terminators (Perkin-Elmer, Applied Biosystems Division, Foster City, CA, USA), and reaction products were analyzed with an ABI 3100 (Perkin-Elmer) automatic sequencer.

The recovered DNA samples were subjected to sequencing with the matching T11N anchoring primer, using a cycle sequence technique in the presence of a radiolabeled terminator (USB Corp., Cleveland, OH, USA).

Purified products were sequenced in separate reactions with each M13 primer using a cycle sequence of 96C, 10 sec; 50C, 5 sec; 60C, 4 min with BigDye® Terminator Mix v1.1 (Applied Biosystems, Foster City, CA).

PCR products of positive samples were sequenced directly by using a dideoxynucleotide cycle sequencing method with an automated DNA sequencer (ABI PRISM 377; Perkin-Elmer).

After reamplification with the same set of primers the PCR products were sequenced on a semi-automated sequencer (ABI 3100, Applied Biosystems) using a Taq cycle sequencing kit (Applied Biosystems, Villebon Sur Yvette, France).

Sequencing of the purified amplicons was performed using a Big Dye Terminator cycle sequencing kit V3.1 (Applied Biosystems) and an Applied Biosystems 3130XL Capillary DNA Sequencer machine.

Sequence analyses of the purified DNAs were performed using a Big Dye Terminator cycle sequencing kit V3.1 (Applied Biosystems) and an Applied Biosystems 3130XL Capillary DNA Sequencer machine.

The D310 repeat sequencing was performed on a Gene Amp PCR System 9700 (Applied Biosystems) using a Big Dye Terminator cycle sequencing kit (Applied Biosystems) as described previously (Lievre et al, 2005).

The amplicons were then cycle-sequenced in both directions using an ABI cycle sequencing kit (Applied Biosystem 3130).