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Data extraction, plate normalization and well annotation were carried out using a customized database interface.
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Phylogenomic analyses used a customized database combining sequences from the NCBI non-redundant (nr) database, dbEST, the Taxonomically Broad EST Database (TBestDB) [ 31] and additional eukaryotic genomes.
To examine repetitive sequences in both the HSY and the corresponding X, the sequences were masked by RepeatMasker using a customized repeat database as a library consisting of Repbase, TIGR repeat data, and papaya repeats [ 20].
The references retrieved from the searches were downloaded into Endnote X6 and were managed using a customized Access 2010 database.
All candidates were individually scored and the final list of hits entered into a tight junction proteomics database using a customized filemaker table (see Supplement figure S2).
We performed double data entry using a customized Filemaker-Pro (Filemaker-Pro, Version 6, Santa Clara, CA) database.
DGE tag sequences together with the corresponding counts, normalized scores, their ranks, etc. were uploaded to the GBrowse2 MySQL database, and they are shown in the browser using a customized version of the Bio::Graphics::Glyph::xyplot module.
All statistical tests were performed using a customized R script.
Next the linker sequences were identified and trimmed from individual reads using a customized Perl script.
Single, and ten cell implantation was performed using a customized glass capillary pipette with manual aspiration.
We treated 123 metastatic non-squamous cell lung carcinoma patients using a customized approach.
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