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Fingerprints are indexed using a custom index type.
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We aligned reads, using Bowtie 1 (Langmead et al., 2009), first to an index containing an unmutated mrps16 sequence, and then the unaligned reads were aligned using a custom-built index containing all possible deletions of the mrps16 exon that could be generated using our combinatorial, outward-facing PCR strategy.
For sequencing, 250-bp paired-end reads were used with a custom index primer (5′-AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG-3′); sequencing was performed to an estimated coverage of 61× per base.
Repeat-induced point mutation (RIP) indexes were calculated for 500-bp sliding windows across the genome for both N. tetrasperma strains, using a custom Perl script and the composite index described in Lewis et al. (2009).
The phase sensitive SPR system using a custom CMOS camera gave an optimum refractive index sensitivity of 3×10−7 RIU within a refractive index range of 2×10−2 RIU.
Indexed reads were deconvoluted using a custom Perl script (GenXPro GmbH).
A standard P-E model [103] was optimized to data using a custom written routine following [104] to determine key parameters of photosynthesis, including the light utilization index, maximal photosynthesis (Pmax) and light saturation index (Ek) of the P-E curves as defined by Sakshaug et al. [105].
Indentation testing was performed using a custom built test rig.
XPS spectra were obtained by using a custom designed spectrometer.
Experiments are conducted using a custom designed PV array simulator.
The coefficient of thermal expansion for polymer concrete was measured using a custom built device.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com