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BAC end sequences (BES) in this physical map were masked with RepeatMasker v 3.2.7 [28], using a custom database which integrates repeats from Repbase [29] and plant repeat databases [30] and RetrOryza [31].
Patients were followed from 1990 to 2013 using a custom database.
The draft genomes were then annotated with Prokka v1.7 (Seamann 2014) using a custom database containing CD630 CDSs and visualized using Artemis v15.0.0 (Rutherford et al. 2000).
The gene expression matrix for hierarchical clustering were generated by using a custom database [ 45, 46] (filter: more than two-fold changes in at least one of the tested time points).
We decided to analyze repetitive regions that the BLASTN search identified as 300 bp or longer and had an E-value of 0, by subjecting them to a BLASTX search using a custom database of plastid genes from closely related species.
Recent pathway-based, rather than gene-based, analyses using a custom database of genes curated from existing literature for preterm birth (dbPTB), have implicated several potentially relevant pathways that can be used to elucidate further gene-gene and gene-environment interactions [ 52, 57].
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MG-RAST by default allows the direct use of several 16s rRNA databases (LSU, SSU, M5RNA, RDP, and Greengenes) [ 36], but it is not possible to use a custom database.
Analysis of repetitive sequence in BES was performed using a custom repeat database combined with RepBase database version 20061006 [ 28].
The primers were tested in silico using a custom made database including all entries in the RDP with taxonomic information and longer than 1400 nucleotides.
Using a custom relational database developed in house, cDNA libraries were collected and divided into 2 pools (endothelial and non-endothelial cells respectively) and formatted into BLAST databases.
Repeats annotation was performed with RepeatMasker (v. open-3.3.0) using a custom redundant database available from SolGenomics website (ftp://ftp.solgenomics.net/tomato_genome/repeats/).net/tomato_genome/repeats/
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