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Murine brains were removed and frozen in solid CO2, sectioned using a cryostat, mounted on slides and allowed to air dry.
Subsequently, 40 50 μm frozen coronal sections were cut using a cryostat, mounted on gelatine-coated glass slides and stained with cresyl violet.
Longitudinal sections (8 μm) were cut at −25 °C using a cryostat, mounted on SuperFrost Plus slides (Menzel Gläser, Braunschweig, Germany), and stored at −80 °C.
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Forebrain tissue was sectioned into 20 µm coronal slices using a cryostat and mounted onto slides.
Sections of 5 µm were cut using a cryostat and mounted on Star Frost adhesive glass slides (Knittelgläser, Braunschweig, Germany).
Brain tissues were cut into coronal sections using a cryostat and mounted on glass slides (Superfrost, Fisher, Loughborough, UK) previously coated with poly-L-lysine (Sigma).
Subsequently, the brains were transferred to 30% sucrose until they sank and were then cut in 25 µm sections using a cryostat, thaw mounted on Superfrost plus slides (3 sections/slide) and air dried for 10 minutes before incubation in blocking buffer containing 0.3% Triton X-100 (in 10 mM Tris-phosphate buffered saline) and 5% normal horse serum.
Coronal sections (30 μm) were made using a cryostat and mounted on glass slides (Matsunami, Japan).
Sections of 4 μm specimens were prepared using a cryostat and mounted on Super Frost Plus slides (Menzel-Gläser).
Blocks of the pellets were cut into 3 μm thick slices using a cryostat and mounted on glass slides.
Sections of 4 μm were prepared using a cryostat and mounted on Super Frost Plus (Menzel-Gläser) slides.
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