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The number of THP-1 cells washed in PBS was counted using a counting slide.
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Chloroplasts were counted using an eosinophil counting slide (Spiers-Levy, Blue Bell, PA).
For growth kinetics in culture tubes, adherent trophozoites were detached at indicated time with ice-cold incubation for 5 min and cells were counted using a Malassez slide and trypan blue exclusion.
The number of cells in samples of lymph was counted using a haemocytometer slide.
Trypan blue dye was added to ASMCs detached and suspended in 1ΧPBS solution and live cells that did not absorb the dye were counted using a Neubauer slide.
Our intersection procedure also provides an efficient alternative to the q-gram procedure, used in SHRiMP (Rumble et al., 2009) and RazerS, which scans the entire genome using a sliding window and counts q-mers within bins to find candidate genomic regions (Rasmussen et al., 2006).
The number of surviving and dead individuals was counted after 24 h using a 1-ml Hawksley counting slide.
The number of occurrences of each of the 185 hexamers was independently counted within a given sequence using a sliding window approach, and the counts for each word were summed to obtain the total number of −10 σ70 sites.
In particular, TruHMM overcomes the aforementioned limitations of the earlier method by allowing a transcript to have highly non-uniform coverage at different positions, and explicitly addressing the uncovered gap problem using a sliding window-based centroid read counting strategy in a pre-processing step.
Using a slide viewer.
Then a Python script using a sliding window of 300 bp to count mapped reads was used to identify where the read coverage peaked.
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