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CA1 subfield was analyzed using a counting frame of 7155.3 μm2.
BrdU-labeled and eGFP cells were counted exhaustively using a counting frame and a sampling grid of 90 × 90 μm.
RSG cells were evaluated using a counting frame of 140 × 90 μm, and a sampling grid (x = 155, y = 155).
Subicular cells were evaluated using a counting frame of 140 × 90 μm, and a sampling grid (x = 155, y = 155).
Cells were counted using a counting frame and a sampling grid of 75 × 75 μm at a magnification of × 63.
Alternative sampling strategies were examined by reducing the area sampled in the XY plane (using a counting frame smaller than the sampling grid), or by reducing the number of sections sampled (Fig. 4 and Supplementary Methods II).
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We used a counting frame of 1874.2 μm2 with step lengths of 78.93 × 78.93 μm.
Cell profiles were counted at a final screen magnification of × 1024 using a 2D counting frame and a simple, unbiased counting rule (Gundersen, 1977).
Fiber intersects were counted using unbiased, systematic sampling using a 60× objective with a counting frame size of 50 µm×50 µm, grid size of 130 µm×200 µm, and virtual plane separation of 7.0 µm [30].
The length density of the myelinated fibers in the cortex was estimated using an unbiased counting frame.
Counts of TH+ varicosities were made at regular predetermined intervals (x = 150 µm, y = 150 µm) using an unbiased counting frame of known area (6 µm×6 µm = 36 µm2).
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