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Cells for the experiments were grown in batch cultures, and growth was monitored by cell counting using a Bürker-Türk counting chamber, counting 4 500 cells per volume-unity.
Photo documentation and cell counting by using a counting chamber was performed at every passage.
The number of THP-1 cells washed in PBS was counted using a counting slide.
Cells were counted using a counting frame and a sampling grid of 75 × 75 μm at a magnification of × 63.
BrdU-labeled and eGFP cells were counted exhaustively using a counting frame and a sampling grid of 90 × 90 μm.
After 7 days, tumorspheres larger than 60 μm in diameter were counted manually using a counting grid.
After a 4-day period of incubation, cells in 24-well cell culture plates were gently washed with calcium-containing Dulbecco's phosphate buffered saline and viable cells were counted using a counting chamber (Dekaney et al. 2008).
When reaching 80% confluence again, cells were trypsinated, washed twice in phosphate buffer saline (PBS) solution and counted using a Burker turk counting chamber.
Subsequently, 100 µl of cell suspension was mixed with 100 µl of a trypan blue staining solution (Biochrom AG, Berlin, Germany) and the living (colourless) and dead (blue) cells were counted using a Bürker cell counting chamber and an inverted microscope (TMS-F, Nikon, Belgium).
Viable cells were counted using a Burker cell counting chamber.
Finally, when the Daphnia died the body length was measured and the concentration of mature parasite spores [ 39] was counted using a Neubauer Improved counting chamber.
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