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Photo documentation and cell counting by using a counting chamber was performed at every passage.
For serial transfer experiments, cultures were grown overnight and total cell numbers were determined microscopically using a counting chamber (ZINTL, Western Germany).
Adherent and floating cells were quantified using a counting chamber.
Spore concentration was determined by using a counting chamber.
Duplicate flasks were trypsinised and attached cells were quantified using a counting chamber.
Spore numbers were estimated using a counting chamber (Neubauer-improved, 0.1 mm, Marienfield).
Similar(43)
Cell proliferation assays were performed by direct counting using a bacterial counting chamber (Hausser Scientific Partnership, PA, USA) and differential interference contrast microscopy.
Subsequently, every cell fraction was counted using a Bürker counting chamber.
Cell numbers of every cell fraction were counted using a Bürker counting chamber.
An aliquot (10 µL) was stained 1∶1 (vol/vol) with a 0.05% erythrosine B-dye solution in PBS and viable cells (uncoloured) were counted using a cell counting chamber.
Subsequently, MNCs were counted using a microscope counting chamber.
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