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Tracheal swabs were collected using a cotton tip applicator and the secretions collected were suspended in 0.5ml saline.
Cells on the upper membrane surface were removed using a cotton tip, and migrated cells (on lower membrane surface) were fixed and stained by Diff-Quick stain kit (Siemens, Malvern, PA).
All Pap smear specimens were obtained by gynaecologists from the endocervix, cervical transformation zone and discharge at posterior fornix of vagina using a cotton tip stick and Ayre spatula, as described in the VCE technique.
Inserts were then washed with PBS (×2) and cells in the upper chamber removed using a cotton tip (Johnston & Johnston, Ire); cells remaining in the underside of the insert were then fixed in methanol (10 minutes), and 0.5 % crystal violet (Cruinn, Ire) was added to the lower chamber to visualize cells that had invaded across the membrane.
After 24 h of culture at 37°C under 5%CO2/95%% air, the cells on the upper surface of the membrane were removed using a cotton tip applicator, whereas the migratory cells on the lower membrane surface were fixed with methanol and stained with Giemsa (Sigma).
Similar(55)
The femur surface was then finely cleansed with a small amount of acetone (M-Prep Neutralizer 5) using a cotton tipped applicator to prevent contamination.
On day 0 and 5 during session 1 and 4, vaginal smears were obtained using a cotton tipped applicator, rotated three times, 2 cm from the vaginal orifice.
The insert was washed several times in water, and the non-migrated cells in the interior of the insert were removed using a cotton-tip swab.
To apply the gel, rinse your mouth thoroughly and apply one or two drops of the gel directly onto the infected area using a cotton applicator tip.
Squeeze a small amount of the glue onto your natural nail, then use a cotton-tipped Q-tip to spread it evenly across the surface.
Honey too, can be applied on the face of the mole using a cotton swab or Q tip.
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