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The dried plant material was ground using a conventional rotor mixer.
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Tissue was immediately homogenized using a conventional rotor-stator homogenizer for 20 40 s.
The induction motor is simulated using a wound rotor machine, and is rated 0.2 MVA.
The collected supernatant was centrifuged using a Beckman rotor Ti 50 at 20,000 rpm for 30 minutes.
All the high speed spins were carried out in a Beckman (L-80) ultra-centrifuge using a 70TI rotor or swinging bucket rotors as described above.
Cells were harvested by centrifugation at 500 × g in a Sorvall centrifuge using a SH-3000 rotor.
Robotic manipulation of the deletion mutant array was conducted using a Singer RoToR HDA (Singer Instruments).
Gradients were centrifuged at 200,000 × g for 3 h at 4°C, using a swinging rotor.
SGA analyses were performed using a Singer RoToR as described in Tong et al. (2004).
Ultracentrifugation was performed using a SW41 rotor at 35,000 rpm for 3h 20 min at 4°C.
qPCR was performed using a Qiagen Rotor Gene Q PCRl-time PCR detector system with SYBR Green PCR Kit (Qiagen, Germany).
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