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Genomic DNA was extracted using a conventional proteinase K extraction and a QIAamp DNA Blood Kit (Qiagen, Valencia, CA) following the manufacturer's protocol and stored at −80°C.
DNA was isolated from frozen tissues or cell lines using a conventional proteinase K and organic extraction method, as previously described [ 42].
The tissue DNA was isolated from 100 mg of corresponding tissues using a conventional proteinase K/organic extraction method as previously described [ 35].
Somatic DNA was extracted from tumour tissue and genomic DNA from peripheral blood (normal control) using a conventional proteinase K-phenol/chloroform protocol (Shen et al, 2000).
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Fixed tissues were processed into paraffin using a conventional protocol.
DNA was extracted using a conventional SDS/proteinase K/phenol method.
After proteinase K inactivation at 95 °C for 10 min and centrifugation at room temperature for 10 min, genomic DNA in the supernatant was recovered and then quantified using a conventional spectrophotometer.
Hyundai's design uses a conventional 6-speed transmission.
DNA was prepared from tissue and blood specimens using the conventional proteinase K digestion and phenol/chloroform extraction method.
Genomic DNA was extracted from 200 μL of whole blood using conventional proteinase K digestion and the phenol/chloroform extraction method.
High molecular weight genomic DNA from both blood and tumour was purified using conventional proteinase K digestion and phenol-chloroform extraction.
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