Sentence examples for using a conventional pcr from inspiring English sources

Individual standards were prepared from gene specific PCR products generated using a conventional PCR machine, electrophoresed on an agarose gel and subsequently extracted and quantified.

Then, specific primers for the T-DNA and specific primers for the adaptor sequence were used to amplify the unknown flanking sequences using a conventional PCR procedure.

This study sought to characterize the genetic background of enterotoxigenic C. perfringens strains, firstly using a conventional PCR survey for representative virulence genes on the chromosome or for genes borne on toxin plasmid(s), as well as for several housekeeping genes.

DNA samples were analyzed by using a conventional PCR (11 ).

Messenger RNA expression for all named TGFβ superfamily ligand members was determined using a conventional PCR block cycler (Hybaid, Middlesex, UK).

Prior to RT-qPCR, the various sets of gene-specific primers were tested using a conventional PCR and run in a 2.5% agarose gel stained with ethidium bromide.

The amplification was performed for 30 cycles using a conventional PCR-approach.

Initially, pandemic (H1N1) 2009 was detected by using a conventional RT-PCR (pH1N1 PCR) specific for the hemagglutinin gene (8 ).

Human Plasmodium species were excluded by using a conventional nested PCR and real-time PCR (8, 9 ).

RNA extracts from pH1N1 PCR negative specimens were frozen at –80°C and, once specimen collection was completed, were retested by using a conventional RT-PCR assay for all influenza A viruses (matrix PCR), which has a higher sensitivity (9 ).

Study design: RNA was extracted from 161 bat brains and the samples were compared using a conventional RT-PCR and the TaqMan based assay.