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The paraffin-embedded samples were cut into 5-μm sections using a conventional microtome.
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The tissue sections (8 μm) were then obtained using a microtome (Leica freezing microtome CM1950, Germany).
Paraffin blocks were sectioned at 5 μm thickness using a microtome (Shandon Finesse Microtome 325®: Thermo Electron Corporation Walthamm, U.S.A).
Then, 8 µm Palaffin sections were made using a RM2255 microtome (Leica).
A total of 6 µm sections were cut using a cryostat microtome (Leica CM1850; Leica Microsystems) and affixed to slides.
The tissues were embedded in a 2 1 mixture of OCT: 30% sucrose and sectioned using a cryostat microtome.
For examination, sections were cut using a freezing microtome.
Granule cryosections, 10 20 µm thick, were obtained at −20 °C using a HM550 microtome cryostat (MICROM International GmbH, Germany).
Thin sections of 4 µm thicknesses are cut using a rotary microtome machine.
Leaf samples were cut using a sliding microtome, stained with Safranin-O and counterstained with Fast Green.
Frozen sections (50 µm) were cut using a sliding microtome.
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