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Correctness of measurements was ensured using a control serum (Calibration sera level 1 3) by Randox Laboratories Ltd. (UK) and urine materials (Liquichek urinalysis level 1 2) by Bio-Rad (USA).
The specificity of this signal was ascertained using a control serum for ChIP.
No IgE-binding spots were detected by immunoblotting using a control serum from a nonallergic subject (result not shown).
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Assay performance was assessed within and across runs using a quality control serum sample processed and spotted multiple times with test samples.
Delamaire et al. used a single control serum for all samples to remove the possibility of a difference in opsonisation [ 61], convincingly demonstrating that no phagocytosis defect exists.
The ROTEM® device was tested regularly for correct function using quality control serum (ROTROL®; Pentapharm GmbH).
CRP was measured by the turbidimetric method with intensification by a particles reaction in the spectrophotometer Cobas Mira® Plus (Roche Diagnostics, Basel, Switzerland), using a calibrator and control serum of Biosystem (Bayer AG, Leverkusen, Germany).
Assay performance was monitored every 50 tests, using the lipid control serum available commercial kit.
Assay performance was monitored after every 25 tests using control serum, Precinorm (cat. no. 1446070; Boehringer Mannheim, Germany) for normal range and Precipath (cat. no. 171778; Boehringer Mannheim) for pathological ranges [ 25].
Reproducibility was demonstrated by 21 replicate determinations using an in-house control serum sample, included among analytical batches.
Phosphate binders are widely used to control serum phosphorus levels in patients with chronic kidney disease (CKD).
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