We have taken this into account in our experiments by using a control cell line (pLKO vector cells) that was cultured in the same puromycin-containing media as our shRNA cells.
In this paper, we assessed the role of the neuronal gene stc in lifespan control using a control fruit fly line, a line with an insertional stc KG01230 mutation, and four lines with precise reversions of this mutation, rev1, rev3, rev4, and rev5.
Two representative RNAi lines were used for each gene in the subsequent analysis and the empty vector (1301) transformed callus was used as a control line.
The Cantony line was used as a control line, completely devoid of any P element or transgene (true "M" line).
For the release, we also selected six independent transgenic citrange lines (designated C1, C3, C4, C5, C6 and C8) and one non-GM regenerant, which was used as a control line (CC).
To investigate the function of SMAD4 in PDAC cells, we made a cell line in which SMAD4 was stably knocked down by performing lentiviral transduction with shRNA against SMAD4 in SMAD4-positive PANC-1 cells, as well as a control cell line using a control construct.
Interestingly, the use of a control line may lead to inaccurate interpretation of the data.
A hairy root line that adapted well to liquid media was selected for use as a control line in subsequent experiments.
Use of a control line, derived from the same base population as the selected line, clearly shows that the sensitivity to a new environment, particularly subtropical climate, is increased by past selection.
