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The kinetics of the particle formation process were assessed using a constant evaporation rate model.
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First, CuO films with thickness 200 nm were deposited on cleaned ITO using high resistive and voltage thermal evaporator (Leybold-Heraeus, A-550 V) under high vacuum at a constant evaporation rate of (0.5 Å/S).
An evaporation system with constant boundary conditions will usually have a constant evaporation rate for a single liquid (one component) with respect to time (Stiver and Mackay 1984).
(25) The linear decrease of the radius corresponds to a constant evaporation rate per surface area and time.
Commercial units use a constant 250 volts.
Solvents were then removed by evaporation using a rotary evaporator.
Acetone was removed by evaporation using a rotary evaporator (IKA, Staufen, Germany).
Bakery waste hydrolysate used in fed-batch cultures was concentrated by water evaporation using a rotary evaporator.
Between each step, the supernatants were pooled, and finally concentrated by evaporation using a centrifugal evaporator (SpeedVac).
Lastly, the total lipid content was obtained by evaporation of ether using a rotary evaporator.
The resulting filtrate was concentrated by evaporation under vacuum at 40°C, using a rotatory evaporator (Buchi Labortechnik AG, Switzerland).
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