Sentence examples for using a conserved region from inspiring English sources

The six primers required for accelerated RT-LAMP were designed using a conserved region in the C-terminus of the coat protein coding region of PPV.

Using a conserved region at the N-terminus as an antigen, we generated polyclonal antisera against synapsin I (Fig. S1A).

Important progress in studying the diversity of phycodnaviruses was enabled by the design of degenerate PCR primers using a conserved region of the polymerase gene of Micromonas viruses [33], since this sequence now serves as a phylogenetic landmark for both metagenomic data and newly characterized NCLDV sequences.

TBlastN analysis, using a conserved region of the peptidase S8 domain as the query sequence, was used to identify all putative SLPs in genome sequences.

Some methods use a conserved region for universal primer design, based on the known viral genomes [9] [11].

For GL2, we used a conserved region between EU826520 - (BnGL2a) and EU826521 (BnGL2b).

Phylogenetic tree of silkworm UGTs was reconstructed by the neighbor-joining (NJ) method using a conserved C-terminal region.

To further explore this latter observation, we measured PHYH levels in human, chimpanzee, bonobo, and gorilla fibroblast cultures (6 different donors per species) via quantitative PCR (qPCR) using primers designed against a conserved region of this transcript.

Molecular detection of HPV, using nested PCR amplification of a conserved region of the HPV L1 gene with the consensus MY09/11 and GP5+/6+ primers, revealed the presence of viral DNA in 91.47% of cases (118/129).

All samples were subjected to Brucella genus amplification using B4/B5 primers that amplify a conserved region in all Brucella species to detect the presence of Brucella DNA as one of the possible causes of the AFI.

Molecular detection of HPV DNA, using nested PCR amplification of a conserved region of the HPV L1 gene DNA with the consensus MY09/11 and GP5+/6+primers, revealed the presence of viral DNA in 91.47% (115 of 129 SCC and 3 of 4 adenocarcinoma).