Sentence examples for using a consensus primer from inspiring English sources

In the Lolium chloroplast genome 80% of the SNPs and Indels found occurred in less than 40% of all tracefiles covering the respective region and could have remained unnoticed using a consensus primer walking approach.

PCR amplification of blaCTX-M, blaSHV, blaTEM, and blaKPC genes in K. pneumoniae and E. coli clinical isolates was performed by using a consensus primer pair specific to each type of β-lactamase as described (20 – 20 ).

The JH4 intronic regions were PCR amplified from genomic DNA prepared from PBMC or CD27+ PBMC (when fresh blood samples were available) using a FR3 consensus primer and a primer upstream of JH5 as described previously [58].

We have adapted this concept to in situ RT-PCR recovery by producing single-stranded cDNAs with complementary flanking 5' and 3' terminal sequences, so that PCR can be performed using a single consensus primer (KZ) to amplify the resultant cDNA templates.

At their first or second obstetric visit (mean pregnancy time 32.1 weeks, rank 30 to 34 weeks), consenting women were tested for cervical HPV-DNA status using a screening consensus primer PCR (see below).

Using common primer sets, such as a consensus primer set of small subunit rRNA gene region, a bundle of PCR products can be amplified from a sample with mixed microbial genomes.

To elucidate sequence information from 1 of the 13 isolates, we used a consensus-degenerate hybrid oligonucleotide primer algorithm to design degenerate PCR primers based on highly conserved amino acid sequences within multiple sequence alignments of all viruses in the subfamily Pneumovirinae (3 ).

The same nucleic acid extracts were used both for the NASBA assays and for HPV DNA detection by PCR, the latter using the consensus primer GP5+/6+ with an enzyme immunoassay readout as previously described (Jacobs et al, 1997; Snijders et al, 2005).

The 16S rRNA gene was amplified by PCR using two consensus primer fD1 and rD1 [ 20].

A 450 bp fragment from the L1 region of HPV-DNA was amplified using the consensus primer MY09/11 as described elsewhere [ 14].

The bacterial reference database included all bacterial 16S rDNA while the viral reference database included all poxvirus sequences that are potentially amplified using the consensus primer pair.