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Intermediate 4 (4.2 mg, 7 μmol) was conjugated with DOTA-NHS ester (6.4 mg, 8 μmol) using a conjugation procedure similar to that for 3. Compound 5 was obtained after HPLC purification using Method-2 (5.5 mg, 5.5 μmol, yield = 79%).
The drugs were loaded onto the surface of nanoparticles using a conjugation strategy based on hydrophobic-hydrophobic interactions with the Pluronic co-polymer used as nanoparticle surface coating.
The drugs were loaded onto nanoparticle surface using a conjugation strategy based on hydrophobic-hydrophobic interactions with the Pluronic co-polymer used as nanoparticle surface coating.
For complementation experiments the resulting plasmid pHNC44 was introduced into a spontaneous streptomycin resistant mutant of CVD110 (resulting in strain HNC44) using a conjugation method described by Datta et al. [26].
We then probed the interaction between H60a and NKG2D in the context of cellular interactions using a conjugation assay.
Fmp peptide was conjugated to carboxyl functionalized IO nanoparticles with diameter of 10 nm (Ocean Nanotech Cat #SHP10) via 1-ethyl-3- 3- dimethylamino propyl) carbodiimide (EDC)/ N-hydroxysulfosuccinimide (sulfo-NHS) chemistry using a conjugation kit (Ocean Nanotech Cat # ICK).
Similar(53)
Using a tailored conjugation chemistry, we generated highly stable selenomab-drug conjugates and demonstrated their potency and selectivity in vitro and in vivo.
We also demonstrated that the distorted phase of the object beam introduced by a image distorter was successfully restored using a phase conjugation mirror.
Using an EDC/NHS conjugation method, collagen was chemically modified to incorporate a photosensitive cinnamate moiety.
Using an EDC/NHS conjugation method, chitosan was chemically modified to incorporate a photosensitive α-cyano-4-hydroxycinnamic acid moiety with various degrees of substitutions.
In this current study, this targeting has been extended to chitosan/alginate nanoparticles using a similar carbodiimide conjugation approach conjugating free carboxyl groups to amino functions.
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