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The samples were stained with three different MitoTracker dyes, similar to Hbt. salinarum, and were examined using a confocal fluorescence microscope.
Indeed, our first experiments showed that living Hbt. salinarum cells can be stained in their growth medium using three MitoTracker dyes (MitoTracker Orange CMTMRos, MitoTracker Red-CMXRos, and MitoTracker Deep Red FM), as described in Materials and Methods and observed using a confocal fluorescence microscope (see Fig. 1).
The samples were then subjected to electrophoresis, stained with DAPI and observed and photographed using a confocal fluorescence microscope.
The slides were then mounted in a mounting medium (Vector Laboratories) and visualized and photographed using a confocal fluorescence microscope (LSM 710, Carl Zeiss International).
The images were examined using a confocal fluorescence microscope (Nikon) and SPOT imaging software (Diagnostic Instruments, Inc., Sterling Heights, MI, USA).
For evaluation of total body wall muscle and neuronal tissue area 5 nematodes of each line were imaged using a confocal fluorescence microscope (LSM 710, Zeiss).
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For the optical setup, we used a confocal fluorescence microscope with a single mode fiber (SMF) for delivering the excitation light from two LDs (Neoark, TC20-4820-4.5 and TC20-4030-4.5) which operate at wavelengths of 488 nm (pump light) and 405 nm (reverse light).
To collect the fluorescence signal in the MSIP, slides were scanned using a confocal laser fluorescence scanner Luxscan-10K/A (CapitalBio, Beijing, China) with a resolution of 10 μm per pixel.
The fluorescence was visualized at 488nm excitation wavelengths using a confocal laser scanning fluorescence microscope (Carl Zeiss LSM510, Axiovert 100 M, Jena, Germany).
The images were captured using a confocal laser-scanning fluorescence microscope Leica SP5 (Molecular Probes, Leica Microsystem) at 63× magnification.
Cell nuclei were stained using DAPI dye for 30 min and the resulting labeled constructs were visualized in confocal fluorescence microscopy using a confocal aperture that corresponded to a back-projected size of 1 airy unit.
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