Using a conditional gene-trap strategy, the authors discovered a novel role of REST in genome protection during neurogenesis.
Thus, we refined our strategy and sought to investigate the significance of Gαs and Gαi2 for physiological heart rate regulation in the sinoatrial node of the adult mouse using a conditional gene-targeting approach.
Hence, because the constitutive knockout was embryonic lethal and the cellular model was inappropriate to study the role of Hsp90b1 in female gametes and early preimplantation embryos, it was necessary to use a conditional gene targeting approach.
We have used a conditional gene-targeting approach to investigate the relative contributions of HIF-1 and HIF-2 to VHL-associated vascular tumorigenesis in a mouse model of liver hemangiomas.
Genes were subsequently filtered using a conditional formula that removed genes with contradictory features and, within each gene, returned fold change values associated with features exhibiting the smallest mean P value.
Here, we used both a conditional gene deletion strategy that targets the deletion of STAT3 selectively to astrocytes (STAT3-CKO), and a pharmacological inhibitor of JAK-2, AG490, in cultured astrocytes in vitro, to investigate potential functions and molecules influenced by STAT3 signaling in relation to mitochondrial function and oxidative stress.
This was done using a conditional hypergeometric test; conditioning means that each term was tested for statistical overrepresentation after removing genes that overlapped a significant child term.
In a first attempt to elucidate the biological function of the Cbx3 gene, we undertook a gene-targeting experiment using a conditional targeting vector.
conditional ama1 gene knockout mutants have been obtained in P. berghei and T. gondii using a conditional FLP/ FRT system (Bargieri et al., 2013).
To re-examine the role of REST during embryonic neurogenesis, we use mice containing a conditional gene trap (GT) cassette in an intron of the endogenous Rest gene that terminates transcription upstream from the initiator codon.
