Sentence examples for using a conditional co-transfection from inspiring English sources

Using a conditional co-transfection system we were able to rescue the infectivity of both the HIVNC-N and HIVNC-C to 72 % and 59 of the HIVwt infectivity while retaining enough TC tagged NC proteins for visualization by fluorescence microscopy.

Our data shows that this conditional co-transfection system can be used to rescue the infectivity of selected HIVTC viruses.

To determine whether co-expression of CIN85 with hTTP affected the latter's ability to promote destabilization of a TNF-based RNA transcript, we used a co-transfection assay described previously [8].

To predict conditions of peroxisome proliferation using biclusters, we used conditional co-expression features to build a classifier to predict conditional dynamics of peroxisome proliferation.

Using the reporter co-transfection assays, we showed that ectopic expression of βTrCP1 resulted in a significant (∼40%) decrease in FOXO3 transactivational activity (Figure 2E).

His6 -GFP was used for co-transfection to monitor transfection and pull-down efficiency.

SUR2A was co-transfected into a COS1 line stably expressed a Kir6.2 pore [ 20] using a TransIT-COS transfection kit (Mirus, Madison WI).

All cells were co-transfected with renilla vector (50 ng) using Fugene Transfection Reagent (Roche, Basel, Switzerland) using a DNA/Fugene transfection reagent volume ratio of 1 3.

For SSA assays, 0.5 μg TALEN expression plasmid and SSA reporter were co-electroporated into 5.0 × 105 HEK293 cells using a Neon transfection system according to the manufacturer's instructions (Life Technologies).

Evaluate using a co-signer.

Using co-transfection experiments, we found that Aur-A protein was degraded via Cdh1, but not Cdc20 (Figure 2A), as previously reported [18], [19], [23], [24], [28].