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The amount of p-nitrophenol released was quantified using a concentration plot of a p-nitrophenol standard.
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The refractive index of each reservoir solution was measured before and after a 37 day incubation period and the final PEG 4000 concentration determined using a plot of refractive index versus PEG 4000 concentration (7% to 20% (w/v)) calculated using Prism version 4 (GraphPad Software, California, USA).
On a biaxial system where x-axis was the serum concentration of the cytokine and y was optical density, the sera cytokine concentration was determined using a curve plotted based on the optical density of known serial concentrations.
Readings were converted to corresponding concentrations using a standard calibration plot.
This means that cheaper conductivity measurements can be applied without losing significant accuracy, and the measured values can be converted into salt concentration using a calibration function (plotted in Fig. 6).
Half-maximal inhibition (IC50) by fosmidomycin was determined using a plot of enzyme fractional activity as a function of inhibitor concentration.
The extract concentration providing 50% inhibition (IC50) was calculated using a graph and plotting inhibition % against extract concentration [ 21].
Kinetic constants were determined using Hanes plots from experiments carried out using a fixed inhibitor concentration of 20 mM xylose at varying PNPX concentrations (0.5 - 4 mM).
The half-maximal inhibitory concentrations (IC50) values were estimated from the AA% versus concentration plots using a non-linear regression algorithm.
The amount of laser scatter caused by insoluble particulates (relative nephelometry units, RNU) was plotted against compound concentration using a segmental regression fit, with the point of inflection being quoted as the compounds′ aqueous solubility (in μ m).
The amount of laser scatter caused by insoluble particulates (relative nephelometry units) was plotted against compound concentration using a segmental regression fit, with the point of inflection being quoted as the aqueous solubility.
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