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To further improve the distribution of chain lengths, a separation method based upon weight was applied using a concentration gradient.
Some experimental results from the literature are difficult to explain by the existing diffusion theories using a concentration gradient or a swelling rate.
Using a concentration gradient it was found that higher concentrations of gefitinib were needed to inhibit ERK signalling (IC50 of 394 and 356 n M, respectively) as compared to the receptor phosphorylations (IC50 of 22 and 84 n M, respectively) in the NR6wtEGFR and NR6M cell lines (Table 2, Figure 3B and D).
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Subsequent IP assays that used a concentration gradient of ABL1 demonstrated that synbody 9 had high affinity for ABL1, Kd = 12 nM (Figure 5D).
They use a concentration gradient to find the odor source.
We assume that D. melanogaster only uses a concentration gradient to find the odor source and that dispersal can be modeled with a two-dimensional chemotaxis model for the redistribution of flies.
Adsorbed proteins were eluted using a linear concentration gradient of NaCl (0 0.5 M) at a flow rate of 0.5 ml/min.
Elution using a stepwise concentration gradient was able to produce fractions of reduced diameter population from the polydisperse HiPCO starting material, where a good correlation between the concentration of elution and local bond curvature for each nanotube species was observed.
The primer pairs were validated if they matched the following conditions: PCR efficiency range between 80 100% as determined using a DNA concentration gradient (40ng-20ng-10ng-5ng-2.5ng), Cycle thanshold (Ct) lower than 30 cycles, and all pathological genotypes detected in the validation set.
Peptides were eluted using a continuous concentration gradient of ammonium chloride in the presence of 0.1% formic acid and 30% acetonitrile.
His6-tagged TssK was eluted using a linear concentration gradient of 0 500 mM imidazole in IMAC elution buffer B (50 mM Tris/HCl, pH 7.5, 250 mM NaCl and 500 mM imidazole) over 12 column volumes.
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