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To overcome the limitations of manual assessment, the BSI methodology was automated using a computerized image analysis system that employed an artificial neural network [5].
Optical density was measured at 20× magnification using a computerized image analysis system (ImageJ, NIH, MA, http://rsb.info.nih.gov/ij/).
Analysis of microglial cell density was performed using a computerized image analysis program (Image-Pro Plus, Media Cybernetics, Inc., Silver Springs, MD).
Using a computerized image analysis system (NIH image, version 1.61), the area of infarction was measured at the sides of the inner section.
Optical density was measured at ×100 magnification using a computerized image analysis system (ImageJ, NIH, MA, http://rsb.info.nih.gov/ij/) that read optical density as grey levels.
The area of the region of interest was measured and, after background correction, the number of immunopositive nuclei was quantified using a computerized image analysis system (Leica Qwin version 2.3, Leica Microsystems Imaging Solutions).
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HuC/D-immunoreactive neurons stained dark brown and were counted in submucous and myenteric ganglia on longitudinally-cut sections using a computerized, image-analyzing system (Imagescope).
Measurements of the density of silver grains over tissue profiles were performed using a computerized image-analysis system (NIH image, version 1.61).
Measurements of wall layer thickness were performed on deparaffinized, hydrated, and hematoxylin-eosin-stained paraffin sections from the uterus by using a computerized, image-analyzing system (Imagescope, Aperio ScanScope GL SS5082, Vista, CA 92081, USA).
The western blots were evaluated quantitatively using a computerized digital image system LAS-40000 mini; GE Healthcare, Tokyo, Japan).
The optical density (OD) of the autoradiographic images was measured using a computerized PC-based image analysis system (NIH Image).
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