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Analysis of the individual comets to generate quantitative data was performed using a computer based program ([34], VisComet; Impuls Computergestutzte Bildanalyse GmbH, Gilching, Germany).
Patients were randomly assigned to two groups using a computer based program.
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To retain as much of the temporal response information as possible we used a variant of a computer based program called FeelTrace, Figure 1 [19].
Randomization was performed with a computer based program at the beginning of the trial and no blocks were used since this is not viable in our study design.
The off line analysis was performed using a computer based image analysis program (CapImage®, Engineering Office Dr. Zeintl, Heidelberg, Germany).
Participants were assigned to treatment in accordance with the schema at Figure 3 using a computer based, stratified block randomisation program to ensure that within each stratum the treatment groups were of virtually equal size.
In order to identify putative cis-acting elements that regulate p120ctn promoter, we used a computer based DNA sequence analysis program known as AliBaba2, a software program for predicting binding sites of transcription factors in an unknown DNA sequence utilizing the TRANSFAC database [ 8].
Fractal dimension was measured from baseline photographs using a computer-based program following a standardized protocol.
Retinal vascular caliber was measured from baseline photographs using a computer-based program following a standardized protocol.
Retinal vascular caliber was measured using a computer-based program following a previously validated protocol (16, 17).
Quantitative changes of retinal vascular caliber and arteriolar bifurcation geometry were measured using a computer-based program.
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