Images of ethidium bromide-stained gels and western blots were scanned and analyzed by densitometry using a computer based on the Sigma Gel System (SPSS).
Off-line analysis was performed using a computer-based image analysis program (CapImage®, Engineering Office Dr. Zeintl, Heidelberg, Germany).
Optical density of immunoblots was measured using a computer-based image analysis system (MCID Analysis, St Catharines, Ontario, Canada).
Autoradiographs were analyzed using a computer-based image analysis system (AIS, Imaging Research Inc., Ontario Canada) that converts transmitted optical density to the amount of radioligand bound in pmol per microgram of protein.
Hippocampal area both on the left and right sides was measured using a computer-based image analysis system (Image J, NIH, Bethesda, MD, USA), and areas from 8 sections per a mouse were summated.
Regions of interest were outlined with a light pen, measured, and CREB or pCREB positive nuclei were counted using a computer-based image analysis system LEICA (LEICA Imaging System Ltd., Cambridge, England).
Digital video images were recorded using a personal computer based system consisting of a WebCam (PC-Cam 300, Creative Labs, Milpitas, CA) and WebCam Control Center version 5.6 software.
To facilitate this, the concept map was projected onto a screen using a computer image projector.
The pictures were analyzed using a computer-assisted image analyzer (Olympus Microimage™ Image Analysis, software version 4.0 for Windows, USA).
Adipocytes size measurement was performed using a computer-assisted image analysis progres® capture pro software.
