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The root tip was then squashed, mounted onto a glass slide, and examined using a compound photomicroscope.
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Individual anthers were then removed and macerated in 60% glacial acetic acid, stained in aceto-orcein [46], spread under a coverslip by warming over a naked flame, and observed using a Zeiss Photomicroscope III.
Vascularity was photographed using a dissecting photomicroscope (Leica, WILD macroskop).
Images of the stained blood vessels were taken using a Leitz photomicroscope.
Sections were examined using a Zeiss photomicroscope (Carl Zeiss Inc., Thornwood, NY, USA) equipped with a 3-chip charge-coupled device colour camera (DXC-960 MD; Sony Corp., Tokyo, Japan).
This analysis was performed at 200x magnification using a Nikon photomicroscope (Microphot-FXA) connected to a KS-300 apparatus (Kontron Elektronic, Germany).
Photographs were taken by using a photomicroscope (Labomed, India) attached to a camera (Nikon D70).
Photomicrography of the stained samples was done using a photomicroscope (OPTIKA B-350).
The lower surfaces (with migrant cells) were captured using a photomicroscope (5 fields per chamber) (BX51 Olympus, Japan), and the cells were counted blindly.
At 24 h of incubation, images (10×) of the same areas were recorded using a photomicroscope (BX51 Olympus, Japan), and closure of the wounds was processed using Image-Pro Plus 6.0.
Graphs were randomly taken from the stained slides using a Zeiss Axiophot photomicroscope (amplification: 20×).
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