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As it will be discussed later, strains in the web was measured at each side by using a complete rosette of ten linear variable displacement transformers (LVDT).
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At this developmental stage, Arabidopsis possesses a complete rosette growth and an initial emergence of inflorescence.
Next, GUS activity was measured using a total rosette protein extract to minimize the characteristic patchy tissue distribution effect of areas with different infection levels.
Sampling was accomplished using a Conductivity, Temperature and Depth (CTD) probe Sea-Bird Electronicss, Bellevue, WA) and a rosette equipped with 10-liter Niskin bottles fitted with silicone closure springs.
Genomic DNA was isolated from rosette leaves using a DNeasy Plant Maxi kit (Qiagen, Hilden, Germany).
Genomic DNAs were extracted from fresh tissue obtained from young rosette leaves using a modified CTAB extraction protocol [ 52].
Complete their installation by using a wrench.
The glucosinolate content of the rosette leaf material was further quantified using a dedicated HPLC analysis.
Rosette leafs of 8-week-old short-day-grown plants were flash frozen in liquid nitrogen, grinded using a swingmill (Retsch, MM301) and resuspended in protein extraction buffer.
A total of 2096 bottle samples were obtained using a CTD/Rosette sampler during 57 cruises (1995 2014) in the range 116 121.5°E and 22 26.5°N (see Supplementary Fig. S2).
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