The mean 25-hydroxyvitamin D level was 80% higher when using a competitive protein-binding assay as compared with HPLC while intermediate values were found with a RIA assay.
We tested 200 serum samples by using a competitive viral protein 7 (VP7) ELISA Institute Pourquierr, Montpellier, France).
Serum oestradiol was analysed on the Roche E170 analyser (Roche Diagnostics GmbH, Mannheim, Germany) using a competitive chemiluminescence immunoassay after release from binding proteins by mesterolone.
Binding to the human DR4 protein was also evaluated using a competitive ELISA-based assay.
Ceruloplasmin protein concentrations were measured using a competitive enzyme-linked immunoabsorbent assay (ELISA) as per the manufacturer׳s instructions (AssayMax Human Ceruloplasmin ELISA Kit EC4001 1, Assaypro LLC, St. Charles, MO, USA).
The ability to bind the antibodies by native proteins and their hydrolysates was determined using a competitive ELISA test.
MGP protein concentrations in cell culture supernatant were quantified using a competitive MGP ELISA kit following manufacturer's instructions (Biomedica, Vienna, Austria).
The binding of glycopeptides 1 21 to Aq was evaluated using a competitive ELISA-based assay in which the glycopeptides, in a range of concentrations, were incubated with recombinant soluble Aq protein and a fixed concentration of a biotinylated marker peptide.
Binding affinities were also estimated using a competitive displacement radiolabel receptor assay as previously described (Keyt et al, 1996) but substituting VEGFR2 ΔKD m for the KDR-IgG fusion protein, and using the fusion proteins secreted from insect cells.
Using a competitive assay with glutathione, we also explored the potential transfer of Pt to biological nucleophiles and particularly to DNA in protein-containing methionine-Pt adducts.
