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Second, we screened the pool from the first stage using a competitive phage ELISA, to identify genuine pointed-end binders.
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IgG against BTV was detected by using a competitive ELISA (Pourquier Bluetongue Competitive ELISA; Pourquier Laboratory, Montpellier, France).
After four rounds of panning, several phage clones were tested for their binding ability with TBBPA by a competitive phage ELISA, and the optimal one was selected for the remaining studies.
We first used a competitive binding assay.
Statistical tests conducted use a competitive null hypothesis.
Furthermore, we show that by using a helper phage (Phaberge) that assembles phage particle preferring the Cry1Ac-pIII fusion protein, display of Cry1Ac was further improved (Fig. 1a).
isolated using a similar phage display approach.
The phage particles were then assembled using a helper phage (Stratagene, VCS M13, 10 cfu/ml).
Other companies, led by Cambridge Antibody Technology Group and MorphoSys, are producing fully human antibodies using a technique called phage display.
To screen serum biomarker of knee osteoarthritis (OA) using a phage random peptide library.
The composition of the polystyrene binding peptide domain was identified using an M13 phage display library.
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