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Using a competitive assay with glutathione, we also explored the potential transfer of Pt to biological nucleophiles and particularly to DNA in protein-containing methionine-Pt adducts.
Two biomarkers of degradation were measured in urine samples: CTX-II (C-telopeptide of type II collagen) using a competitive assay (CartiLaps; IDS) and C2C-HUSA (collagenase-generated peptides of human type II collagen) using a sandwich assay (IBEX) (Cibere et al. 2009).
Vaccine-induced anti-HPV GMTs were measured using a competitive assay, which measures the antibody response to known neutralising epitopes.
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Next, we have prepared a small library of PACAP-based analogs and evaluated the pharmacological profile of a few of them using a competitive binding assay, as well as functional and survival assays.
Analogs were tested for binding to I-Aq using a competitive binding assay and selected analogs were administered to arthritic mice.
Using a competitive binding assay, Est-en had 28% relative binding affinity (RBA) for the ER as compared to 17β-estradiol.
The insulin contents of the ISG fractions were measured using a competitive ELISA assay.
Duration of receptor binding is expressed as the dissociation constant (koff) of the receptor-ligand complex, measured using a competitive radioligand assay.
Using a competitive binding assay as described in the Methods section, signal decreases of 59%and13%3% indicated positive response to InO and G544, respectively.
By using a competitive binding assay, here we demonstrated that the technetium tricarbonyl indeed binds selectively to a His-tagged protein in the presence of a non-His-tagged protein and little or no (<2% ± 0.9%) non-specific labelling was observed.
We investigated the effects of TBMEHP on deiodinase activity using a competitive substrate assay developed by Butt et al. (2011).
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