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The targeting ability of PA-PEG1k-RGD was further established using a competition assay.
The binding of the small-molecule CXCR4 antagonist AMD3100 to CD4/CXCR4-proteoliposomes was analyzed by FACS using a competition assay [47], [55].
The binding of the CXCR4 ligand CXCL12 to CD4/CXCR4-proteoliposomes was analyzed by FACS using a competition assay [46], [55].
After the silica particles were removed by centrifugation, the concentration of the monosaccharide in the supernatant was determined using a competition assay as described in our previous publication.
The Zn(ii) affinities (K Zn values) of Atx1 and PacSN have previously been measured using a competition assay with the chromophoric chelator RhodZin-3 (Table 1).
The binding affinity of enzalutamide to AR WT and AR F876L, relative to dihydro-testosterone (DHT), was determined using a competition assay in which increasing concentrations of cold competitor are added to cells preincubated with F-FDHT.
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We have now used a competition assay to assess the efficiency of tRNA-aminoacylation of these analogs.
We therefore used a competition assay to confirm that a mutated hinge binds less efficiently.
For example, in our study we used a competition assay, which would not identify those TFs that binds to both methylated and un-methylated DNA motifs equally well.
Therefore, we used a competition assay in which TTP plasmas were preincubated with and without 10 nM MDTCS in solution (to preabsorb IgG recognising these domains) prior to incubation with wells coated with full-length ADAMTS13.
For each potential nonameric binding frame, we synthesized a 12 15 residue peptide containing the region of interest in its natural context, and determined the binding affinity using a competition fluorescence polarization assay (Figure 6a).
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