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The quantitative RT PCR data were analyzed using a comparative threshold (Ct) method, and the fold inductions of the samples were compared with the untreated samples.
The relative levels of mRNA expression were calculated using a comparative threshold cycle (Ct) method.
Fold expression was calculated using a comparative threshold cycle method (2-Δ ΔCT) [ 16].
Fold changes in expression of each gene were calculated using a comparative threshold cycle (Ct) method with the formula.
The relative expression of IL3 was calculated using a comparative threshold cycle method, using the Equation 2^ -ΔΔCt).
The relative expression of TRAIL mRNA (using the same primers indicated above) was calculated by using a comparative threshold cycle method and the formula 2− ΔΔCt).
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Data was analyzed using a comparative cycle threshold (ΔCt) method [ 32].
The relative gene expression of targets was detected using a comparative cycle threshold method [ 20].
The qRT-PCR data was analyzed using a comparative cycle threshold (ΔCt) method [ 21].
Gene expression was quantified using a comparative critical threshold (CT) method as described previously [ 20].
The qRT-PCR data was analyzed using a comparative cycle threshold (∆Ct) method.
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