Total RNA was extracted using a commercial kit (miRNeasy microKit, Qiagen, Hilden, Germany).
Intracellular staining for IL-10 (Bioss) was performed using a commercial kit for cellular fixation and permeabilization (BD Biosciences).
Phospholipid content was determined using a commercial kit (Wako Chemicals).
Ribonucleic acid (RNA) was isolated using a commercial kit (RNeasy mini kit, Qiagen Inc., Valencia, CA).
TRFR was measured by ELISA using a commercial kit (RAMCO, ATI Atlas, Chichester, UK).
RNA from adult mouse tissues was isolated using a commercial kit and protocol (Qiagen).
Immunoglobulins from selected mAbs were isotyped using a commercial kit (Amersham Bioscience, England).
After incubations, inorganic phosphate was immediately measured using a commercial kit (Labtest Diagnóstica, Brazil) [43].
Importantly, the time required for purification was much less than that required using a commercial kit.
Plasma sphingomyelin was analyzed using a commercial kit (Cayman Chemical, Ann Arbor, Michigan, USA).
The total RNA was extracted from A375SM and reverse-transcripted (RT) using a commercial kit (Clontech).
