Sentence examples for using a commercial extraction from inspiring English sources

Using a commercial extraction kit (QIAGEN, Valencia, CA, USA), we extracted DNA from randomly chosen aliquots (1.5 mL) of >50 homemade ice samples obtained from local vendors in Cascavel and performed a standardized nested PCR assay (T. gondii B1 gene, sensitivity 1 parasite).

RNA preparation was undertaken using a commercial extraction kit.

Ten millilitre of active enrichment culture samples were taken from the serum bottles (same bottles as for chemical analysis) and centrifuged at 12,000×g for 10 min and the settling materials on the bottom were used for genomic DNA extraction by using a commercial extraction kit (Axygen Biosciences, USA) according to its instructions.

The pVS plasmid was simultaneously digested with HindIII and AflII, the enzymes heat inactivated at 70°C for 20 min, the DNA phenol chloroform extracted, and the product cut out after electrophoresis on a 1% agarose gel using a commercial extraction kit (Qiagen, catalog no. 28706).

PCR amplification of microsatellites D11S534, D11S970, D13S267, D13S171 and D17S1322 was undertaken on DNA extracted from thin sections of fixed tissue (both tumour and normal lymph node from the same patient), using a commercial extraction kit (Nuclear Biosciences).

Stool specimens collected from 700 individuals were filtered, mechanically lysed twice, and incubated overnight with proteinase K prior to DNA extraction using a commercial DNA extraction kit.

Total RNA extraction was performed using a commercial RNA extraction kit (A&A Biotechnology) according to the manufacturer's protocol.

The one negative dust sample that was obtained was also extracted using a commercial DNA extraction kit.

Genomic DNA from TB samples and cells was extracted using a commercial DNA extraction kit (DNeasy Blood & Tissue Kit, Qiagen, Valencia, CA, USA).

A single colony from each ESBL-producing isolate was transferred into 100  μL of distilled water and the bacterial DNA was extracted by using a commercial DNA extraction kit (RTA, Ankara, Turkey).

Once DNA capture probes were immobilized on optical fibres, we demonstrate their putative application as genosensors in detecting Sus Scrofa DNA in purified samples obtained first using a commercial DNA extraction kit and thereafter using our improved extraction method.