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CGRP was determined by using a commercial enzyme immunoassay.
Pretreated sugar cane bagasse was further submitted to enzymatic hydrolysis using a commercial enzyme complex formed by cellulases and β-glucosidases.
Peripheral visfatin levels were measured using a commercial enzyme immunoassay kit with a measurement range from 0.1 1000 ng/ml.
Synthesis of cDNA was allowed to proceed for 2 h at 42°C using a commercial enzyme and RT-PCR kit (QIAGEN).
Fecal calprotectin concentrations were determined in duplicate at 2-week intervals, using a commercial enzyme linked immunoassay (Calprest®, Eurospital, Trieste, Italy).
Serum ferritin (sFr) and serum transferrin (sTfr) were estimated from plasma samples using a commercial enzyme linked immunosorbent assay kit (Spectro Ferritin Kit, Spectro Transferrin Kit; Ramco Laboratories, Inc., Houston, Texas).
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S100A6 serum concentrations were analyzed using a commercial enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's instructions (SEB769Hu by Cloud-Clone Corp., Houston, TX, USA).
VDBP concentrations (milligrams per liter) were measured in duplicate using a commercial enzyme-linked immunosorbent assay among healthy African American (n = 56) and Caucasian American (n = 60) participants.
We have obtained a written informed consent from the patient and healthy volunteers for publication of this case report and measurement of plasma OXA concentrations before and 1 h after anesthesia induction and at emergence from anesthesia using a commercial enzyme-linked immunosorbent assay kit (Peninsula Laboratories, San Carlos, CA).
After 24 h, blood samples were collected, and plasma was harvested by centrifuging blood at 13,800× g for 15 min. Plasma levels of hemopexin were measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Life Diagnostics, West Chester, PA, USA), according to the manufacturer's instructions.
TGF-β1 protein levels were measured using a commercial enzyme-linked immunoassay (ELISA) (Bioscience) following the manufacturer's instructions.
More suggestions(15)
using a commercial extraction
using a commercial expert
using a specific enzyme
using a simplified enzyme
using a commercial refrigeration
using a bacterial enzyme
using a commercial cost
using a commercial standard
using a recombinant enzyme
using a commercial package
using a commercial software
using a colorimetric enzyme
using a particular enzyme
using a fluorometric enzyme
using a commercial code
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